Papers

et-I

Rosensweig C, Reynolds KA, Gao P, Laothamatas I, Shan Y, Ranganathan R, Takahashi JS, Green CB. An evolutionary hotspot defines functional differences between CRYPTOCHROMES. Nature Communications. 2018 Mar 19;9(1):1138.

In this paper, we show that mammalian cryptochrome (CRY) proteins share an allosteric mechanism with the distantly related photolyase enzymes that mediate DNA repair after UV damage. The regulatory site has been modified in the CRY proteins to differentially recognize proteins that modulate the mammalian circadian clock. This work adds to the general concept that despite great divergence in cellular functions, allosteric mechanisms are often deeply conserved in protein families.

et-I

Subramanian SK, Russ WP, Ranganathan R. A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components. Synthetic Biology. 2018 Jan 18;3(1):ysx008.

An important technical goal is efficient, high-throughput construction of synthetic genes. Here, we report a useful resource towards this goal – a validated set of 166 20-nucleotide orthogonal PCR primers. Used in pairwise combination, it is possible to create 13,695 gene-specific primers, each capable of uniquely amplifying the sequences corresponding to individual genes from commercially available large mixed pool oligonucleotide syntheses.

et-I

Subramanian SK, Russ WP, Ranganathan R. A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components. Synthetic Biology. 2018 Jan 18;3(1):ysx008.

An important technical goal is efficient, high-throughput construction of synthetic genes. Here, we report a useful resource towards this goal – a validated set of 166 20-nucleotide orthogonal PCR primers. Used in pairwise combination, it is possible to create 13,695 gene-specific primers, each capable of uniquely amplifying the sequences corresponding to individual genes from commercially available large mixed pool oligonucleotide syntheses.

Publications